Comparative Genome Analysis of Genes Regulating Compound Inflorescences in Tomato.

Yahui Yang,Huanhuan Yang,Jingbin Jiang,Tingting Zhao,Yinxiao Tan,Xiangyang Xu,Jingfu Li

doi:10.3390/ijms222212548

Abstract

Inflorescences are the main factor affecting fruit yield. The quantity and quality of inflorescences are closely related to fruit quality and yield. The presence of compound inflorescences in cherry tomatoes is well established, and it has been discovered by chance that compound racemes also exist in tomatoes. To explore the formation of compound inflorescences in tomato, transcriptome sequencing was performed on Moneymaker (MM) and Compound Inflorescence (CI) plants. In-florescences were collected in three periods (early, middle and late) in three replicates, for a total of 18 samples. Data analysis showed that the DEGs were most enriched in metabolic pathways and plant hormone signal transduction pathways. The DEGs were also enriched in the cell cycle pathway, photosynthesis pathway, carbon metabolism pathway and circadian rhythm pathway. We found that the FALSIFLORA (FA), COMPOUND INFLORESCENCE (S) and ANANTHA (AN) genes were involved in compound inflorescence development, not only revealing novel genes but also providing a rich theoretical basis for compound inflorescence development.

Highlights

In-florescences were collected in three periods in three replicates, for a total of 18 samples
At least 92% of the reads were mapped to the tomato reference genome, and more than 90% of the clean reads were among the mapped reads
The results showed that the Differentially Expressed Genes (DEGs) mainly regulated the hormones indoleacetic acid (IAA), abscisic acid (ABA), brassinosteroids (BR), ethylene, cytokinin (CTK), jasmonic acid (JA), salicylic acid (SA) and gibberellin (GA)

Summary

Introduction

In-florescences were collected in three periods (early, middle and late) in three replicates, for a total of 18 samples. Many important genes involved in the regulation of inflorescence development have been identified, such as the SELF-PRUNING (SP) [13], FALSIFLORA (FA) [14], COMPOUND INFLORESCENCE (S) [15], ANANTHA (AN) [16], UNIFLORA (UF) [17], TERMINATING FLOWER (TMF) [18], SINGLE FLOWER TRUSS (SFT) [19], MACROCALYX (MC) [20], JOINTLESS (J) [21], BLIND (BL) [22], and LATERAL SUPPRESSOR (LS) genes [23] Many transcription factors, such as LFY, AP1, TFL1, SOC1, FA, SFT, AN, TMF and S, play important roles in the developmental regulation of the inflorescence meristem and flower meristem in both Arabidopsis thaliana and tomato [24,25,26]. AN and FA are mainly expressed in the flower meristem and control the formation of the flower meristem [19,31]

Methods

Results

Discussion

Conclusion