Abstract
BackgroundGoose parvovirus (GPV) causes acute enteritis, hepatitis, myocarditis and high morbidity and mortality in geese and ducks. GPV H strain was isolated from a Heilongjiang goose farm where the geese were showing signs of hemorrhage in the brain, liver, and intestinal tract. In this study, we explored the genetic diversity among waterfowl parvovirus isolates and the pathological characteristics of GPV H in Shaoxing ducklings.MethodsThe complete capsid protein (VP) and non-structural (NS) sequences of the isolated H strain were sequenced, and phylogenetic trees of VP and NS were constructed in MEGA version 5.05 using the neighbor-joining method. Three-day-old Shaoxing ducklings were inoculated with GPV and were euthanized at 1, 2, 4, 6, and 8 days post-inoculation (PI), and their organs were removed and collected. The organs of 6-day PI ducklings were fixed in formalin, embedded in paraffin, sectioned for histology, stained with HE and analyzed for pathological lesions. The distribution of the GPV H strain in the tissues of the inoculated ducklings was detected using the polymerase chain reaction (PCR) method.ResultsGenetic analysis of the NS and VP genes indicated that the H strain was closely related to strains circulating in China during 1999–2014, and the nucleic acid identity of those strains was 98%–99%. Classical symptoms were observed in the inoculated ducklings. GPV remained in many tissues and replicated in a majority of the tissues, leading to histopathological lesions in four tissues.ConclusionsWe first reported the distribution and histopathological lesions of a Chinese strain of GPV in infected shaoxing ducklings. This H strain was moderate pathogenic for Shaoxing ducklings.
Highlights
Goose parvovirus (GPV) causes acute enteritis, hepatitis, myocarditis and high morbidity and mortality in geese and ducks
Many phylogenetic analysis have indicated that the GPV strains of mainland China can be divided into three distinct groups, PRC-I, PRC-II and PRC-III, and novel goose parvovirus (N-GPV) was closely related to these GPV strains [11]
We explored the pathogenicity of the H strain in 3-day-old Shaoxing ducklings by analyzing clinical symptoms, pathological changes, and the distribution of GPV in the tissues of ducklings inoculated with the virus
Summary
Goose parvovirus (GPV) causes acute enteritis, hepatitis, myocarditis and high morbidity and mortality in geese and ducks. GPV causes watery diarrhea, weight loss, and stunting in ducklings and goslings, with high morbidity and mortality, and this disease is known as 3-week disease or Derzsy’s disease [1, 2]. The genome contains two main open reading frames (ORFs), with the GPV circulated in the Fujian, Jiangsu, Heilongjiang, Shanghai, Guangdong, Sichuan and Shandong Provinces in China during 1999–2014 [7, 8]. Since 2015, many Cherry Valley ducklings flocks appeared short beak and dwarfism syndrome in Northern China, including Jiangsu, Anhui, and Shanghai Provinces, and this disease was caused by a distinct GPVrelated virus, namely novel goose parvovirus (N-GPV) [9, 10]. Many phylogenetic analysis have indicated that the GPV strains of mainland China can be divided into three distinct groups, PRC-I, PRC-II and PRC-III, and N-GPV was closely related to these GPV strains [11]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
