Abstract
<p class="AbstractSummary"><strong>Objective: </strong>To evaluate for single nucleotide polymorphisms (SNPs) within the tenascin-C (TNC) gene in a population of dogs with atraumatic Achilles tendon rupture.</p><p class="AbstractSummary"><strong>Background:</strong> In humans, Achilles tendinopathy has been extensively studied for numerous polymorphisms within several genes and has been associated with polymorphisms in collagen (COL5A1) and the TNC genes.</p><p><strong>Evidentiary value:</strong> This study serves as a starting point for evaluating a genetic component of Achilles tendinopathy in the dog.</p><p><strong>Methods: </strong>Whole blood from twenty dogs with atraumatic Achilles tendon rupture and 14 matched control samples were used. DNA was extracted from whole blood run with primers designed around two SNPs previously identified to be related to Achilles tendinopathy in humans. One SNP was located in exon 29, and one exon 17 of the canine TNC gene. Polymerase chain reaction (PCR) was run on the samples and they were sequenced. Sequences of the affected canine population were compared to the control sample sequences.</p><p class="AbstractSummary"><strong>Results:</strong> There were no significant differences in genotype or allele frequency of the SNPs rs13321 and rs2104772 between any of the affected and control subjects with a <em>p</em>-value of 1.0.</p><p class="AbstractSummary"><strong>Conclusion: </strong>This study evaluated a population of canines with atraumatic Achilles tendon rupture for SNPs in the TNC gene. We found no difference in gene sequence for the study population compared to age, sex, and breed matched controls.</p><p><strong>Application:</strong> Though the data from this study did not show a correlation between the specific polymorphisms investigated, it is possible that other SNPs within the TNC gene or other genes involved in tendon composition and repair such as collagen may be associated with atraumatic Achilles tendon injury in the dog. </p><br /> <img src="https://www.veterinaryevidence.org/rcvskmod/icons/oa-icon.jpg" alt="Open Access" /> <img src="https://www.veterinaryevidence.org/rcvskmod/icons/pr-icon.jpg" alt="Peer Reviewed" />
Highlights
The common calcanean tendon, or Achilles tendon, is comprised of three main components; the paired tendon of the gastrocnemius muscles; the combined tendon of the gracilis, semitendinosus, and biceps femoris muscles; and the tendon of the superficial digital flexor muscle
In humans, Achilles tendinopathy has been extensively studied for numerous polymorphisms within several genes and has been associated with polymorphisms in collagen (COL5A1) and the TNC genes
This study evaluated a population of canines with atraumatic Achilles tendon rupture for SNPs in the TNC gene
Summary
The common calcanean tendon, or Achilles tendon, is comprised of three main components; the paired tendon of the gastrocnemius muscles; the combined tendon of the gracilis, semitendinosus, and biceps femoris muscles; and the tendon of the superficial digital flexor muscle. Tendons are exposed to high mechanical stresses, and disruption can be partial or complete. Intrinsic risk factors for developing tendinopathy, including genetic variations in chromosome 9, are well documented in humans. Achilles tendinopathy has been extensively studied for numerous polymorphisms within several genes and has been found to be associated with polymorphisms in collagen (COL5A1) and the tenascin genes[4,7,8,9,10,11,12,13,14,15,16]. Achilles tendinopathy has been extensively studied for numerous polymorphisms within several genes and has been associated with polymorphisms in collagen (COL5A1) and the TNC genes.
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