Comparative Gel Electrophoresis of Soluble Proteins and Enzymes of Rice Blast Fungus Pyricularia oryzae Cav.

Abstract

One hundred and thirty-two isolates of the rice blast fungus (Pyricularia oryzae Cav.) of various sources were tested. Soluble proteins extracted from mycelia and enzymes from culture filtrates were subjected to the polyacrylamide gel disc and thin layer electrophoresis. Two isolate groups were distinguished in the soluble protein patterns and peroxidase zymograms, but these have no significant correlations with the geographical distribution of the isolates or their pathogenicity.In the non-specific esterase zymograms, 3 types were distinguished and the types showed significant correlations with the geographical distribution of the isolates. The taxonomic and phytopathological significance of these findings were discussed.