Comparative GC-MS analysis of the composition of metabolites of aboveground and underground parts of Comarum palustre L

Abstract

Introduction. Understanding the mechanisms of accumulation of individual groups of biologically active substances in promising types of plant raw materials and the possibility of predicting them is important for solving fundamental and applied problems of pharmaceuticals. To date, differences have been revealed in the qualitative and quantitative composition of secondary metabolites in the aboveground and underground of Comarum palustre L., however, the issue remains unstudied.Aim. Comparative metabolomic study of the composition of the primary metabolites of the aboveground and underground parts of Comarum palustre L.Materials and methods. The object of the study was the aboveground and underground parts of Comarum palustre L., harvested in the vicinity of the nursery of medicinal plants of the St. Petersburg State Chemical and Pharmaceutical University (Leningrad Region, Vsevolozhsky District, Priozerskoe Highway, 38 km) in 2019 and dried. Metabolomic studies based on GC-MS method was perfomed. A statistical analysis based on the MetaboAnalyst 5.0 platform was used.Results and discussion. Analysis of the chromatograms obtained using the GC-MS method revealed the content of 933 primary metabolites in the aboveground and underground parts of Comarum palustre L., 120 of which were identified. Using a number of statistical methods, 10 metabolites from monosaccharides, acids and alcohols, making the greatest contribution to the manifestation of differences between the studied samples, were identified.Conclusion. The study revealed the relationship between the composition of primary and secondary metabolites in medicinal plant raw materials.