Comparative Feasibility of Oxidative Stress and Immune-Inflammatory Response Induced in the Secondary Intermediate Host by Different Viability Status of the Hydatid Cysts.

Abstract

Echinococcosis is a common health problem in the Mediterranean and the Middle East. Many hydatid cysts remain asymptomatic, even in advanced age due to the slow growth of the parasite. The present study aimed to investigate the oxidative and inflammatory responses in rats' echinococcosis induced by three different viability statuses of the Echinococcus granulosus (G6) as diagnostic markers. Forty-eight male albino rats were injected intraperitoneally with three different viability statuses of the hydatid cyst fluid of the camel strain. The groups included: the negative control group (1), the low viable protoscoleces (2), the high viable protoscoleces fluid (3) not viable and not completely transformed to the calcareous status of protoscoleces fluid (4). Serum was harvested at the end of each week from the 9th to the 12th week post-infection for measuring the oxidative stress by total antioxidant capacity (TAC), and lipid peroxide (Malondialdehyde) (Malondialdehyde or MDA). Splenic tissues from different groups were collected for histopathological examination. The results showed a histopathological change, a significant decrease in TAC levels, and an increase in malondialdehyde, the TNF-α, and IL-10 levels of the infected groups compared with the uninfected group (P<0.05). Our study concluded that echinococcosis induced severe oxidative stress and inflammatory responses including tissue necrosis and tissue degeneration are the factors that can be used in the early stages of infection, avoiding hazards of contamination.