Abstract
BackgroundThe present study was conducted to assess the efficiency of using TK SLC-L (Salubris, Inc.) for the primary isolation of mycobacteria from clinical samples by comparing it to the MGIT detection system (Becton Dickinson Diagnostic Instrument Systems). Although TK SLC, a biphasic medium, has been evaluated previously, this is the first study to evaluate TK SLC-L, a liquid medium.MethodsClinical specimens from a total of 146 clinically suspected cases of tuberculosis were studied. Each processed sample was evaluated by ZN staining and inoculated into TK SLC-L and MGIT tubes. The TK SLC tubes were incubated in a MYCOLOR TK while the MGIT tubes were incubated in a MGIT system. Growth, indicated by automated systems, was confirmed through production of a smear and microscopic evaluation after ZN staining.ResultsMycobacterial growth was positive in 35 TK SLC-L and in 34 MGIT samples. Although the growth detection time was approximately 3 to 5 days shorter, on average, with the MGIT system, the contamination rate was significantly lower using TK SLC-L. The total time spent for the repetition of cultures for contaminated samples in MGIT make the total return time for culture results equal to or longer than the time required by TK SLC-L.ConclusionsThe TK Culture System using TK SLC-L is an efficient system and possible alternative to other rapid mycobacterial culture systems.
Highlights
The present study was conducted to assess the efficiency of using TK SLC-L (Salubris, Inc.) for the primary isolation of mycobacteria from clinical samples by comparing it to the MGIT detection system (Becton Dickinson Diagnostic Instrument Systems)
The TK SLC-L and MGIT systems showed a similar percentage of culture positivity (24.0 and 23.3%, respectively)
TK SLC-L (Salubris, Inc.), a liquid version of the biphasic medium TK SLC, was introduced to the market. It enables the detection of M. tuberculosis complex (MTC) in clinical samples based on colourimetry
Summary
The present study was conducted to assess the efficiency of using TK SLC-L (Salubris, Inc.) for the primary isolation of mycobacteria from clinical samples by comparing it to the MGIT detection system (Becton Dickinson Diagnostic Instrument Systems). Given its global prevalence, devastating morbidity, and massive mortality, tuberculosis (TB) has been among the most important of human infections for many centuries. The rapid diagnosis of Mycobacterium tuberculosis is essential to implement adequate antimicrobial therapy and effective disease control. Liquid automated culture systems, which offer high sensitivity for the early diagnosis of TB, are important tools. Middlebrook broth is a commonly used medium in the rapid mycobacterial culture system MGIT (Becton Dickinson Diagnostic Instrument Systems, Towson, MD, USA) and in other systems such as the BacT ALERT 3D (bioMérieux, Marcy l’Étoile, France) and ESP Culture System II
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