Comparative Evaluation of the Antioxidative and Antimicrobial Nutritive Properties and Potential Bioaccessibility of Plant Seeds and Algae Rich in Protein and Polyphenolic Compounds

Abstract

Spice plants are not only a source of nutrition compounds but also supply secondary plant metabolites, such as polyphenols. Therefore, their bioaccessibility is an important issue. In order to understand the biological activity of polyphenols present in spice plants, it is necessary to broaden knowledge about the factors influencing their bioaccessibility, including nutritional factors. Therefore, the objective of this research was to determine the antioxidative and antimicrobial nutritive properties and potential bioaccessibility of plant seeds and microalgae rich in protein and polyphenolic compounds. Plant seeds rich in protein—i.e., black cumin, milk thistle, fenugreek, almonds, white sesame, white mustard, eggfruit and the two most popular algae, chlorella and spirulina—were analyzed for total polyphenolic compounds (TPC) and antioxidant properties (ABTS, FRAP), as well as their potential bioaccessibility, antimicrobial activity, basic chemical composition and amino acid profiles. With regard to the TPC, the highest levels were found in star anise, followed by milk thistle, white mustard and fenugreek, whereas the lowest were noted in white sesame, almonds, eggfruit, spirulina and chlorella. White mustard and milk thistle showed the highest antioxidant capacities and almonds, eggfruit, spirulina, and chlorella the lowest according to the ABTS and FRAP assays. The widest spectrum of microbial growth inhibition was detected for fenugreek extract, which showed antimicrobial activity against four analyzed microorganisms: B. subtilis, P. mirabilis, V. harveyi and C. albicans. The protein from seeds of black cumin, milk thistle, white mustard and eggfruit and chlorella was not limited by any essential amino acids. Among all analyzed plants, fenugreek seeds were judged to have potential for use in food formulation operations in view of their antioxidant activity and amino acid profile. Based on the results, intake of polyphenols together with protein in plant seeds does not have a major impact on the potential bioaccessibility of a range of polyphenols and phenolic metabolites.