Comparative evaluation of solvent extracts of Azanza garckeana fruit pulp on hormonal profiles, spermiogram and antioxidant activities in rabbit bucks

Abstract

The study investigated the comparative influence of different extraction solvents on spermiogram, hormonal profiles and antioxidant activities in rabbit bucks. Adult New Zealand White rabbit bucks (n=18), with average live weight of 1.2±0.03 kg and aged 10-18 mo were fed ad libitum on a commercial diet. They were administered five different Azanza garckeana (AG) fruit pulp extracts at 500 mg/kg via oral gavage, comprising control group (Con), crude (AG Cr), methanol (AG M), n-hexane (AG H), ethyl acetate (AG E) and aqueous (AG AQ) for four weeks. The extracts improved the spermiogram in rabbit bucks administered methanol (AG M) and the reaction time was significantly (P<0.05) lower in AG E group when compared to other groups. The ejaculate volume, sperm motility, pH and sperm concentration were significantly (P<0.05) higher in the AG M group when compared to the other groups. There was a significant (P<0.05) increase in concentrations of blood testosterone, follicle-stimulating hormone and luteinising hormone in methanol extract group (AG M). While the glutathione and malondialdehyde concentrations were (P<0.05) lower, catalase and superoxide dismutase activities were significantly (P<0.05) higher in the groups administered methanol extract (AG M). It was concluded that AG M extracts of AG pulp elicited the best response in spermiogram, hormonal concentrations and antioxidant activities in New Zealand White rabbit bucks. Its use as the extraction solvent is recommended.