Comparative Evaluation of Salivary Glutathione Peroxidase levels in Subjects with Generalized Stage III Periodontitis before and after Periodontal Therapy – A Biochemical Study

Abstract

Introduction To assess the levels of glutathione peroxidase in the saliva of subjects with generalized stage III periodontitis before and after periodontal treatment. Material and Methods This study, compares salivary glutathione peroxidase levels in generalized stage III periodontitis subjects before and after therapy. A total of 20 participants were selected. The clinical and biochemical evaluation was done at specific time intervals as described. The data was collected and analyzed. Levels of dental plaque and gingival index were recorded at baseline and 2 months post periodontal therapy. Salivary glutathione peroxide levels were assessed by ELISA kit at baseline and 2 months post periodontal therapy. Results At baseline, the mean plaque index scores were found to be 2.5585 ± 0.06685 (SD). After 2 months, the values were found to have reduced to 0.9080 ± 0.04224. At baseline, the mean gingival index scores were found to be 2.5475 ± 0.3380 (SD). After 2 months, the values were found to have reduced to 0.8700 ± 0.1676. At baseline, the mean glutathione peroxidase levels were found to be 5.1595 ± 0.3713 (SD). After 2 months, the values were found to have reduced by 4.4040 ± 1.1142 (SD). Conclusions Glutathione peroxidase level was elevated in disease conditions and is associated with the free radical formation in tissue inflammation. Following periodontal therapy, glutathione peroxidase levels are lowered significantly, indicating reduced tissue inflammation. Key-words: Antioxidants, Biomarkers, ELISA, Glutathione Peroxidase, Saliva