Comparative evaluation of salivary F2-isoprostane in type II Diabetic patients with non-diabetic individuals

Abstract

Brief Background Oxidative stress is increased in diabetes mellitus due to excessive production of oxygen free radicals and a deficiency in antioxidant mechanism. F2-isoprostanes are primary end products of lipid peroxidation and are considered to be a biological marker for assessing oxidative stress in tissues. This study aims to evaluate the level of salivary F2-isoprostane in type II diabetic patients and compare it with non-diabetic individuals. Materials and Methods A total of 150 subjects were included in the study comprising of 50 patients with controlled diabetes (Group I), 50 patients with uncontrolled diabetes (Group II) and 50 non-diabetic individuals (Group III).We analyzed the salivary F2 -isoprostane in these subjects using a sandwich ELISA technique. The data was analyzed using SPSS software version 16.0. One way ANOVA was used for the comparison of salivary F2-isoprostane levels among the study groups and Scheffe multiple comparison analysis was used to compare the level of isoprostane between the groups. P-value of less than 0.05 was considered to be statistically significant. Results Salivary F2-isoprostane levels were significantly higher in patients with uncontrolled diabetes compared to controlled diabetes (p<0.01). Non-diabetic individuals showed lower levels of salivary F2 isoprostanes compared to diabetic patients and were found to be statistically significant (p<0.01). Discussion Salivary isoprostanes are reliable markers to assess oxidative damage in patients with diabetes mellitus and hence anti oxidants can be initiated at an early stage to prevent complications. Summary and Conclusions The result of the study indicates that salivary F2-isoprostane can be used as a valuable marker of oxidative stress in diabetic patients. Key Words Diabetes mellitus, ELISA, F2-isoprostanes