Comparative evaluation of remineralizing efficacy of silver containing fluoride, nano hydroxyapatite and self assembling peptide on early enamel carious lesions under atomic force microscope

Abstract

Aim: To evaluate and compare the efficacy of 3 different materials on remineralization of early enamel carious lesions. Materials and Methods:80 maxillary single rooted premolars were divided into four groups(n=20). Group 1: Control,Group 2 : Silver diamine fluoride ,Group 3 : Nano-hydroxyapatite and Group 4: Self assembling peptide. Each group except control group were subdivided into two subgroups. All the materials were demineralized in demineralizing solution for 96 hours, which was daily renewed after 24 hours until uniform early carious lesion were formed. After the formation of lesions, half of specimens from each tested subgroups had the material applied and the half of the specimens didn’t. All the specimens were immersed together in 20 mL of artificial saliva at 37ċ for 24 h. After 24 hours experimental materials were rubbed off using cotton pellate socked in acetone. All the specimens were then washed with distilled water and again immersed in fresh artificial saliva for 48 hours at 37ċ in an incubator. Saliva was exchanged after 24 hours with fresh saliva. The pair of negative control group was only immersed in artificial saliva for 24 and 48 hours continuously and saliva was not exchanged during this period.