Abstract
In the present study a comparative evaluation microscopic agglutination test with rLigB protein based latex agglutination test was carried out, which is a cross reacting lipoprotein able to detect acute infection caused by any pathogenic leptospiralserovars. It was employed for serodiagnosis of leptospirosis. The 46 KDa 6X His tagged LigB protein, obtained by IPTG induction of recombinant E. coli M15 cells containing the N-terminal region of LigB gee in PQE30 expression vector, was purified by Ni-NTA affinity chromatography and adsorbed on latex bead surface for performing latex agglutination test against Leptospirosis suspected wildlife field sera. A total of 80 wildlife sera samples were collec ted, including 27 wild feline sera samples (18 tigers, 8 lions, and 1 jaguar) obtained from Chhatbir zoo, Chandigarh, 42 sera samples (8 tigers, 4 lions and 6 leopards, 2 cheethals, 1 black buck, 12 buffaloes and 9 zoo staff) were received from Jodhpur zoo Rajasthan, 8 sera samples (4 tigers, 3 leopards, 1 lion) from Van Vihar National park, Bohpal, Madhya Pradesh and 3 sera samples (2 lions and 1 tiger) received from Biwani Mini zoo, Haryana, India. The result showed that sera were tested positive by rLigB based LAT, which were reconfirmed using microscopic agglutination test (MAT). The results from LAT were in concordance with MAT. In conclusion, rLigB based LAT is a rapid, pen site, reliable diagnostic tool of high sensitivity and specificity, under laboratory and field conditions, for the detection of Leptospirosis.
 Asian J. Med. Biol. Res. September 2020, 6(3): 440-448
Highlights
Leptospirosis is considered as an important reemerging, ubiquitous zoonotic disease worldwide, both developing and developed countries and the incidence of which is uncertain and it is underestimated (Faine, 1982; Vijayachari et al, 2008)
The time kinetics for the recombinant LigB protein expression showed that detectable amount of recombinant protein was present in SDS PAGE starting from two hours after induction and after eight hours maximum amount of LigB protein was detected in SDS PAGE (Figure 3)
It has been confirmed that recombinant LigB protein as an immunodominant protein against which antibodies are produced in the host during the active infection and anti LigB antibodies are present in sera of infected animals in higher concentration
Summary
Leptospirosis is considered as an important reemerging, ubiquitous zoonotic disease worldwide, both developing and developed countries and the incidence of which is uncertain and it is underestimated (Faine, 1982; Vijayachari et al, 2008). The risk of infection is occurring due to contact with contaminated environments, infected wild animals as well as with synanthropic animals and rodents (Tilahun et al, 2013). The disease can be directly transmitted through contact with secretions, blood or urine of infected animals, or indirectly through water contaminated mainly with urine of reservoir animals (Bharti et al, 2003). Global warming that leads to extreme weather events such as cyclones and floods, increased rainfall, and increased world population and urbanization are considered as the factors associated with the upsurge in the incidence of leptospirosis, as well as the magnitude of outbreaks (Hartskeerl et al, 2011).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
