Comparative Evaluation of Prooxidant/Antioxidant Balance in Seed Progeny of Plantago major L. from Radioactively and Chemically Contaminated Areas

Abstract

There are many studies addressing plant responses to radioactive and chemical contamination of soils, but few works have been devoted to comparison of biological effects in the areas affected by these human-induced factors. Ionizing radiation and heavy metals have different mechanisms of interaction with biota. Both factors, however, are capable of increasing the generation of reactive oxygen species, which cause enzyme malfunction and cell structure damage. The efficiency of antioxidant systems plays an important role in plant resistance to these impacts. The present study offers a comparative evaluation of prooxidant/antioxidant balance in seed progeny of Plantago major L. growing in the East Ural Radioactive Trace (EURT), in the zone affected by operation of the Karabash Copper Smelter (KCS), and in the reference sites. Lipid peroxidation was assessed by determining malondialdehyde. Evaluation of the antioxidant system was based on the activities of superoxide dismutase, catalase, and total peroxidase, and on the content of low-molecular-weight antioxidants. The study showed that the prooxidant and antioxidant statuses of seed progeny of P. major from the contaminated sites were different from the reference samples and from each other. The pooled EURT sample exhibited a prooxidant shift relative to the reference samples, i. e. not only malondialdehyde but also activities of superoxide dismutase and catalase and the content of low-molecular-weight antioxidants were higher than in the reference samples. Malondialdehyde content in seedlings from the KCS zone did not differ from the reference values; superoxide dismutase and catalase activities were decreased whereas peroxidase activity was higher compared to the activities of these enzymes in the reference samples. Thus, the differences in the plant adaptive responses to ionizing radiation and heavy metals are caused by the dissimilarities in the induction of reactive oxygen species