Abstract

Ceiba pentandra and Parmotrema perlatum lichen are two illustrious spices. Apart from their benefit as aromas, These are known for various therapeutic activities. Both are well known as appetizers. Decoctions of theses powders are used to relieve cough, anorexia and helminthiasis etc. Though several studies on antimicrobial activities of these two plants are available, studies on resistant microorganisms and anti tubercular activity are very limited. Hence, the author made an attempt to identify the phytochemical constituents present in methanolic and n-hexane extracts of Ceiba pentandra and Parmotrema perlatum using phytochemical tests and GC-MS method then to evaluate antibacterial activity against 5 resistant microorganisms by microtitre broth dilution method using ciprofloxacillin & streptomycin as reference standards. Methanolic extracts of Parmotrema perlatum and Ceiba pentandra had shown a considerable antibacterial activity than n-hexane extracts and exhibited 90% growth inhibition against H37Rv resistant strain of mycobacterium at 80 & 320 micrograms/ml respectively. N-hexane extracts of both species were found to be poorly effective against H37 Rv.

Highlights

  • Medicinal plants serve as a rich source for various therapeutically active molecules

  • The Gas Chromatography-Mass spectroscopy (GC–MS) analysis [13] of the components were carried by equipping Mass Selective Detector (MSD) (HP6890 GC and HP7673) autosampler, operated at 65eV using acquisition scan mode with HP-5MS at 100 ̊ C oven temperature held initially for 1 min and increased to 280 ̊C by 20 ̊C/min and held for 10 min. 250 ̊C was the injector temperature, and Helium was used as the carrier gas at a constant column flow rate of 1.5 ml/min

  • The anti-tubercular activity of test samples PP(N)E, CP(N)E, PP(M)E and CP(M)E were assessed against M. tuberculosis using Microplate Alamar Blue Assay (MABA)

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Summary

INTRODUCTION

Medicinal plants serve as a rich source for various therapeutically active molecules. Indian spices are wellknown remedies to treat gastrointestinal problems including helminthiasis, GIT infections etc These spices are known to contribute flavor to food and dispense therapeutically active molecules to the body. Though several synthetic anti-bacterial drugs are available, antimicrobial resistance is the serious and most concern problem for today’s global health It has become an emerging field of research and a challenge to the medicinal chemist to discover new molecules with a safe therapeutic index. Though cephalosporins are the effective antibiotics to treat various forms of colon infections, recent studies reported the resistance [5] of Clostridium pathogens against newer cephalosporins. It occurs throughout northern and southern hemispheres. The author had focussed the present study on phytoconstituents and biological activities of Parmotrema perlatum

Collection of Plant Material
Methods
GC-MS analysis
Determination of MIC 90 by micro titre broth dilution method
RESULTS AND DISCUSSION
GC-MS Study
Antibacterial Activity
Anti Tubercular Activity

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