Comparative evaluation of mineral trioxide aggregate, endoseal, and biodentine in furcation perforation repair: A bacterial leakage study

Abstract

Objectives: The aim of this study is a comparative evaluation of the sealing ability of mineral trioxide aggregate (MTA) Angelus, pozzolan-based Endoseal MTA, and Biodentine when used as sealing materials for furcation perforation. This was done with a bacterial leakage model. Materials and Method: Sixty-four permanent mandibular molars were selected and horizontally sectioned at middle third of the root. Cavities of 2 mm depth were prepared at the root ends. Access cavities were prepared, and the canal orifices and the root end cavities were restored with light cured resin. Perforations of diameter 1.6 mm were created in the center of the pulpal floor using a round bur in a low-speed handpiece. The teeth were randomly assigned to three experimental groups (n = 20). The perforation sites in Groups 1, 2, and 3 were repaired with MTA Angelus, EndoSeal MTA, and Biodentine, respectively. The teeth were inserted individually in an Eppendorf vial which was then placed in a McCartney's bottle containing nutrient broth. The reservoirs were filled with 0.5 ml of Enterococcus faecalis. The system was incubated at 37°C and checked for appearance of turbidity in the nutrient broth for 30 days, and these findings were noted. Level of significance was fixed at P = 0.05, and statistical analysis was done with Chi-square analysis using IBM SPSS statistics 20.0 (IBM Corporation, Armonk, NY, USA). Results: In the time interval of 30 days, all the materials showed contamination to varying degrees (MTA Angelus 8/20 samples, Endoseal MTA 10/20 samples, and Biodentine 7/20 samples). There was no significant difference between the three groups (P > 0.05). Conclusions: MTA Angelus, Endoseal MTA, and Biodentine showed contamination in a 30-day incubation period when used as furcation perforation seal materials.