Abstract

The aim of this study was to compare different techniques in order to achieve a high extraction of phenolic compounds from propolis. For this purpose, it was investigated the use of double maceration (24h at room temperature with continuous agitation at 250rpm), double microwave treatments (1min at 140W) and double ultrasound-assisted extraction (15min at 20kHz) using 70% ethanol. The extraction efficiency was measured based on extraction yield, total phenolic content, flavones and flavonol content, and flavanone and dihydroflavonol content. The ultrasonic extraction had an extraction yield higher than microwave extraction and maceration. The yield of the propolis ranged between samples and between the years of propolis harvesting. Of the twelve quantified phenolic compounds, p-coumaric acid was the most abundant (271.65mg/g propolis).

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