Comparative evaluation of incorporation calcium silicate and calcium phosphate nanoparticles on biomimetic dentin remineralization and bioactivity in an etch-and-rinse adhesive system.

Abstract

This study aimed to evaluate the remineralization potential and bioactivity of adhesives, containing amorphous calcium phosphate (ACP) and calcium silicate (CS) nanoparticles (NPs). In this study, dentin slices (n=60) were prepared and etched with phosphoric acid. Next, they were divided into two groups: pre- and post-immersion in a simulated body fluid (SBF) for three weeks. The two groups were also divided into five subgroups (n=6 per subgroup), including the control (0 wt.% NPs); adhesives containing 1 wt.% and 2.5 wt.% (CS) nanoparticles; and adhesives containing 1 wt.% and 2.5 wt.% ACP nanoparticles. The remineralization potential and bioactivity of the adhesives were evaluated. The shear bond strength of the samples (n=18) was also assessed using a universal testing machine. The present results revealed that the adhesive containing ACP and CS nanoparticles showed bioactivity and remineralization potential without any reduction in the bond strength. The outcomes revealed that Cs and ACP nanoparticles induced mineralization in the dentin and incorporation of these nanoparticles to dentin bonding agents could improve the bio-functionalization of dentin bond. Key words:Calcium phosphate, calcium silicate, fourier transform infrared spectroscopy, scanning electron microscopy, tooth remineralization.