Abstract
Background Platelet Rich Fibrin PRF surgical biological substitute harnesses cells with growth factors and cytokines in platelets for enhanced regeneration and tissue repair. The innovation in the low-speed centrifugation concept is the Advanced-PRF. However the impact of type 2 diabetes mellitus on PRF has not been explored till date. Hence this study was carried out to compare growth factor levels in Leukocyte and Platelet Rich Fibrin L-PRF Advanced- Platelet Rich Fibrin A-PRF and A-PRF amongst diabetic individuals.Methods Venous blood was drawn for the analysis of HbA1c CBC platelet indices and PRF membrane preparation. A total of 90 PRF membranes L-PRF A-PRF and A-PRF from healthy well-controlled and poorly controlled diabetics were subjected to assessment of growth factor release of Vascular Endothelial Growth Factor VEGF Insulin Growth Factor IGF and Fibroblast Growth Factor FGF-21 at days 1 3 and 7. The independent t-test one-way ANOVA and Tukey post hoc test were used to statistically analyze the data.Results A significantly higher platelet index and growth factor VEGF IGF-1 FGF-21 release in diabetics from A-PRF in comparison with A-PRF and L-PRF at days 1 3 and 7 were seen. This was significantly higher in the poorly controlled diabetic group p lt0.05. These findings were found to be positively related to platelet indices and HbA1c levels.Conclusion A-PRF of diabetics demonstrated a higher growth factor release over a period of 7 days suggesting its usage as regenerative material.
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