Abstract
Fibre optic immunosensor for the detection of the antibodies specific for Leishmania donovani in the sera of patients suffering from visceral leishmaniasis has been designed and fabricated. Technique invovles a two-step sandwich-type assay that gives quick analysis and is easy to use. Purified cell surface protein of L. donovani had been immobilized on tapered fibre optic core by covalent coupling. In the first step, treated fibres were incubated with the patient serum for 10 min and then with FITC-tagged goat anti-human IgG in the second step. FITC provided the specific fluorescent signal for detection. No accurate volumetric measurement of serum sample is desirable because of test fibre design and the whole procedure takes just 15 min. Fluorescent signal has been found to be sensitive and specific for Visceral leishmaniasis and correlates well with the conventional serodiagnostic procedures, like ELISA and IFAT.
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