COMPARATIVE EVALUATION OF CREATININE IN SERUM AND SALIVA OF CHRONIC KIDNEY DISEASE PATIENTS

Abstract

Background & objectives: Chronic kidney disease (CKD) is progressive reduction in renal function. Renal excretory function can be assessed by measurement of creatinine concentrations in plasma which illustrates the ltration capacity of the glomerulus. The increased serum creatinine levels in CKD patients create a concentration gradient that facilitates increased diffusion of creatinine from serum into the saliva. Saliva has many advantages as a diagnostic media over serum, which includes non-invasive collection, good patient compliance, minimal risk of contracting infection, repeated sampling possible and is cost effective. The purpose of this study was to estimate and correlate serum and salivary creatinine in patients with CKD and to evaluate use of salivary creatinine as an alternative biomarker to serum creatinine in CKD patients. A total sample of 40 subjects aged 25 -6 Methods: 5yrs, inclusive of both genders comprising 20 cases of healthy controls (Group I) and 20 diagnosed cases of CKD (Group II) were selected. 2ml of blood and unstimulated whole saliva was collected from both the groups. The blinded samples were processed in autoanalyzer using Jaffe's kinetic reaction. The collected data were subjected to Statistical analysis. In the present Results: study we found signicant correlation of serum creatinine and salivary creatinine in both controls and CKD. Serum creatinine (6.21 ±4.02) and Salivary creatinine (0.70± 0.61) were both increased in CKD cases compared to Controls Serum creatinine (0.83±0.21) and Salivary creatinine (0.11± 0.07). Serum creatinine and salivary creatinine were xii both increased as CKD progressed. A sensitivity of 75% and specicity of 90% of salivary creatinine was found. We therefore recommend Conclusion: salivary creatinine as a biomarker in CKD and also as an alternative biomarker to serum creatinine in detecting and staging CKD, for assessing disease progression, therapeutic modalities and in screening of large population