Abstract
Sero-detection of brucella antibodies was done in 300 serum samples randomly collected from aborted goats by the classical serological tests like rose bengal plate test (RBPT), serum tube agglutination test (SAT) and rOMP31-based indirect-Enzyme-Linked Immunosorbent Assay (i-ELISA) and dot-ELISA. The seroprevalence for brucellosis was 22.66, 11.33, 24.33 and 17.33% with RBPT, SAT, i-ELISA and dot-ELISA, respectively. The sensitivity and specificity of different tests varied from 56.25 to 87.50% and from 75.00 to 90.00%, respectively. It was found that rOmp31-based ELISA is the most suitable test with 87.5% sensitivity and 90.0% specificity for detection of brucellosis in goats.
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