Comparative Evaluation of Bactericidal Effect of 810 nm Diode Laser and Sodium Hypochlorite on Enterococcus faecalis in Infected Root Canals of Primary Teeth- An in vitro Study

Abstract

Background Irrigation is one of the important aspects of pediatric endodontics. The present study discusses about the antimicrobial effect of sodium hypochlorite with and without using 810 nm diode laser in primary root canals. Aim To assess and compare antimicrobial efficacy of various irrigating solutions against Enterococcus faecalis with or without using 810 nm diode laser in infected root canals.Methodology A total of 12 freshly extracted human single rooted primary teeth were equally divided into group I n6 and group II n6. E. faecalis strain ATCC 29212 obtained was subcultured on BHI Agar and incubated at 37degC overnight. In all the twelve samples access was opened and sterile insulin syringe was used to inoculate the suspension of 0.01 mL E. faecalis into canals. Following this the samples were incubated for one day in plastic vials under aerobic conditions at 37degC. Cleaning and shaping was done using 21mm K file and 21mm H file respectively and canals were instrumented up to 35 size K and H file using crown down procedure. Copious irrigation with 1.5 sodium hypochlorite was done in Group I. Group II was irrigated with 1.5 sodium hypochlorite and endovac suction was done for one minute. Diode laser was used to perform irradiation in group II and microbiological evaluation was done in both the groups. The data were tabulated and statistical analysis was done.Results Statistically significant difference was observed between the two groups for difference in the micro-biological load.Conclusion Laser induced irradiation with 1.5 sodium hypochlorite showed better antimicrobial efficacy.nbsp