Comparative Evaluation of Antioxidant Reactivity between Ovariectomized and Control Urinary Bladder Tissue Using Ferric Reducing Ability of Plasma and Cupric Ion Reducing Antioxidant Capacity Assays

Abstract

Objectives: In rabbits, ovariectomy has been shown to cause reduced blood flow to the bladder, increased free radical generation, and oxidative damage to the bladder. Thus, the antioxidant status of rabbits may play an important role in the damaging effects of ovariectomy. The present study analyzed total antioxidant content (TAC) in control, 14‐ and 28‐day ovariectomized (Ovx) female rabbit bladders using both ferric reducing ability of plasma (FRAP) and cupric ion reducing antioxidant capacity (CURPAC) assays.Methods: Twelve weight‐ and age‐matched virgin female New Zealand White rabbits (∼ 4.0 kg) were separated into three groups of four rabbits each. Group 1 received ovariectomy surgery for 14 days. Group 2 received ovariectomy surgery for 28 days. Group 3 received sham operations.Results: FRAP did not show any significant differences between either muscle and mucosa or control and Ovx samples. CUPRAC revealed higher TAC in the control mucosa compared to muscle and significantly decreased antioxidant levels in 14‐ and 28‐day Ovx mucosal layers compared to the mucosa of the control rabbits.Conclusion: The CUPRAC method proved to be superior to the FRAP method for accuracy.