Abstract
<p><strong>Objective: </strong><em>Memecylon</em> species is being extensively used in traditional medicine for the treatment of skin disorders and it is proved to possess antidiabetic and anti-inflammatory properties. The present investigation was to study the effect of different solvent extracts of five <em>Memecylon</em> species such as <em>M. umbellatum</em>, <em>M. talbotianum</em>, <em>M. edule</em>, <em>M. malabaricum</em> and <em>M. wightii</em> on antidiabetic and antioxidant effects.</p><p><strong>Methods: </strong>Plant extracts were prepared using soxhlet apparatus using different solvents such as hexane, ethyl acetate, methanol and water and obtained extracts were subjected to antidiabetic (α-amylase and α-glucosidase inhibition assays) and antioxidant (2, 2-Diphenyl-2-Picryl Hydrazyl hydrate<strong> </strong>(DPPH), 2,2-Azino-bis (3-ethyl benzothiazoline-6-Sulfonic acid)diammonium salt<strong> </strong>(ABTS), Superoxide radical scavenging assay<strong> (</strong>SRSA) and reducing power assays) evaluated at different doses.</p><p><strong>Results: </strong>Methanol extracts of all five <em>Memecylon</em> species exhibited effective antidiabetic and antioxidant properties among them methanol extracts of <em>M. malabaricum</em> and <em>M. talbotianum</em> have highest biological activity. For α-amylase IC<sub>50</sub> value for both <em>M. malabaricum</em> and <em>M. talbotianum</em> was found to be 100 and 130 µg/reaction and IC<sub>50</sub> value for α-glucosidase was found to be 6.1 and 7.8 µg/reaction respectively. For DPPH the IC<sub>50</sub>value was found to be 190 µg/reaction, for ABTS 31-39 µg/reaction, for SRSA 950-1200 µg/reaction and for reducing power assay 420-490 µg/reaction respectively.</p><p><strong>Conclusion: </strong>The results indicate that methanol extracts of <em>M. malabaricum</em> and <em>M. talbotianum</em> possess potent <em>in vitro </em>antidiabetic and antioxidant activities compared to other <em>Memecylon</em> species.</p>
Highlights
Oxidative stress is reduced by generating antioxidants from the plant and protects the body against free radical-mediated toxicities [1]
The Diphenyl-2-Picryl Hydrazyl hydrate (DPPH), ABTS, Superoxide radical scavenging assay (SRSA) and reducing power assay of 100-400 μg/reaction for DPPH, 20-80 μg/reaction for ABTS, 320-2560 μg/reaction for SRSA and 250-1000 μg/reaction for reducing power assay was calculated, and the IC 50 value was determined from the linear regression of the percent antioxidant activity against extract
The methanol extracts of five Memecylon species showed the best DPPH, ABTS, SRSA and reducing power activity, ethyl acetate, water extracts showed moderate activity and hexane extracts showed lower antioxidant activity
Summary
Oxidative stress is reduced by generating antioxidants from the plant and protects the body against free radical-mediated toxicities [1]. In the India, the genus is mainly distributed in Western Ghats region represented by about 40 species of which 21 are endemic to peninsular India [4, 5]. The species of this family are small trees or shrubs mainly used in the treatment of skin diseases such as herpes, stomach disorders, snakebite, wounds etc., which is mainly used in traditional medicinal system [6, 7, 8] a few compounds have been investigated and several pharmacological properties have been reported from the crude extracts of Memecylon species [9]. The present study was to determine the in vitro antidiabetic and antioxidant properties of different fractions obtained from Memecylon species such as M. umbellatum, M. edule, M. talbotianum, M. malabaricum and M. wightii
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
