Abstract
Water samples collected simultaneously by Nansen and microbiological bottles from the same layers of the Black Sea contain practically the same numbers of saprophytic bacteria.Sterile collection of water samples with Nansen bottles is possible due to the speed of 1 m/sec during lowering: the friction of water prevents the bacteria from settling on the inner walls of the bottle and thus contaminating the deeper water layers. Bacteria remaining in water drops which soon dry up after emptying a Nansen bottle are exposed to the bactericidal action of its metallic walls.At present a Nansen bottle is the only device that is quite reliable for simultaneous sterile collection of a whole series of water samples from the depths of the oceans.
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