Comparative embryotoxicity of selenite and selenate: uptake in murine embryonal and fetal tissues and effects on blastocysts and embryonic cells in vitro

Abstract

The placental transfer and distribution pattern of selenium (Se) in embryonic and fetal tissues after intravenous injection of Na 2 75SeO 3 (Se 4+) or Na 2 75SeO 4 (Se 6+) has been investigated in mice in early, mid, and late pregnancy by use of whole-body autoradiography combined with gamma-counting. The given doses correspond to 45 μg Se/kg. In order to compare the direct toxicity in early embryonic stages, the two valence forms were studied by using in vitro systems with (1) mouse blastocysts and (2) chick embryo fibrobaasts, differentiating into chondrocytes. The embryonic and fetal uptake after administration of Se 4+ and Se 6+ was almost identical. The placental transfer of Se was restricted but, nevertheless, increased with time after dosing and with progression from embryonic through fetal stages thereby suggesting active placental transfer. The highest uptake in the embryo was observed in the neuro-epithelium while the eye, liver and skeleton dominated the distribution pattern in the fetal period. Selenite (Se 4+) was more toxic than selenate (Se 6+) in the vn vitro system, in which embryonic mesenchymal limb bud cells differentiated into chondrocytes as well as in the blastocyst culture system. The results may suggest a direct effect of Se in the embryo as explanation to reported malformations in experimental studies.