Abstract
Anumber of research groups have recently succeeded in down regulating cinnamyl alcohol dehydrogenase (CAD EC.1.1.1.195) activity through genetic engineering with the aim of modifying lignin profiles in plants. In view of the expected extension of this technology to economically important plants, we have tried to obtain additional information on the best antisense constructs for maximal CAD down regulation using tobacco (Nicotiana tabacum cv. Samsun) as a model system. A comparison of different constructs showed that efficiency was greater with homologous cDNAs (partial or full-length sequence) vs heterologous cDNA. In addition, constitutive promoters were, in our hands, more efficient than tissue (xylem) specific promoters. Although a strong down regulation of CAD gene expression was obtained for different transformants at the in vitro stage, a drop in CAD activity reduction was observed for some of them during development in the greenhouse. These different aspects are discussed in the context of future applications.
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