Comparative efficiency of ammonium sulfate alone and ammonium sulfate-octanoic acid combined methods in the purification potential of antibodies

Ahsan Naveed

doi:10.19045/bspab.2019.80113

Abstract

The use of antibodies in the biopharmaceuticals has been increased from therapeutic to prevention through vaccination. However, the purification processes adopted by the industries make them expensive to use. Ammonium sulfate precipitation is an old and easy way to purify the antibodies from the serum and egg yolks. The purity is somewhat compromised with ammonium sulfate precipitation, therefore, a combination of chromatographic techniques is adopted. Presently, ammonium sulfate alone and in a combination with Octanoic acid was used for the purification of antibodies from caprine serum and the egg yolk. The results of purified antibodies in terms of their yield and purity were compared through statistical analysis. The overall study results indicated a better yield and purity of antibodies from the caprine serum through a combined treatment of ammonium sulfate with Octanoic acid, whereas, ammonium sulfate alone gave a better yield but less pure antibodies from the egg yolk. Ammonium sulfate-Octanoic acid combined precipitation of caprine serum yielded 6.67 mg/mL while sole ammonium sulfate yielded 4.49 mg/mL purified antibodies. In case of IgY Ammonium sulfate-Octanoic acid combined precipitation of yielded only 8.90 mg/mL while sole ammonium sulfate yielded 13.80 mg/mL purified antibodies. A combination of ammonium sulfate and Octanoic acid is a good choice for high yield and purity of antibodies from serum. The purity of antibodies from the egg yolk was enhanced through ammonium sulfate in combination with Octanoic acid but the overall yield was poor. Keywords: Antibodies; Ammonium sulfate; Octanoic acid; Precipitation http://dx.doi.org/10.19045/bspab.2019.80113

Highlights

The use of antibodies in the biopharmaceuticals has been increased from therapeutic to prevention through vaccination
The caprine serum was used for the purification of immunoglobulin G (IgG) proteins through a combined ammonium sulfate-octanoic acid precipitation (AS-Octanoic acid (OA)) and sole ammonium sulfate precipitation
Ammonium sulfate-Octanoic acid (AS-OA) method was used for the purification of serum antibodies and results in terms of high yield and purity of antibodies were similar to the research conducted by Perosa et al, 1990 [16]

Summary

Introduction

The use of antibodies in the biopharmaceuticals has been increased from therapeutic to prevention through vaccination. Ammonium sulfate alone and in a combination with Octanoic acid was used for the purification of antibodies from caprine serum and the egg yolk. The overall study results indicated a better yield and purity of antibodies from the caprine serum through a combined treatment of ammonium sulfate with Octanoic acid, whereas, ammonium sulfate alone gave a better yield but less pure antibodies from the egg yolk. Ammonium sulfate-Octanoic acid combined precipitation of caprine serum yielded 6.67 mg/mL while sole ammonium sulfate yielded 4.49 mg/mL purified antibodies. A combination of ammonium sulfate and Octanoic acid is a good choice for high yield and purity of antibodies from serum. The purity of antibodies from the egg yolk was enhanced through ammonium sulfate in combination with Octanoic acid but the overall yield was poor. A 2mL of egg yolk was diluted 9 folds with distilled water at 4 oC and pH was adjusted to

Results

Discussion

Conclusion