Abstract

UV inactivation of Bacillus thuringiensis var. israelensis ( B. t. i .) toxin is a common problem encountered with any floating formulation against mosquito larvae. An alginate encapsulated, slow-release, floating formulation of B. t. i. , incorporated with UV screens such as acriflavin or methyl green or congo red was prepared and evaluated for larvicidal activity against Culex quinquefasciatus . The data indicated: i. Encapsulation of B. t. i . toxin in sodium alginate has prolonged its residual activity for 45 days, ii. all the UV screens used gave significant level (F=21.66, df = 3716, P<0.0001) of protection to B. t. i . toxin from inactivation by sunlight even after 180 h exposure to direct sunlight (4 hours exposed to direct sunlight daily till 45th day) and iii. compared to acriflavin or methyl green, congo red provided excellent protection.

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