Abstract
BackgroundCadmium poisoning in the environment has assumed an alarming problem in recent years. Effective antimutagenic agents which can reverse or combat cadmium induced genotoxicity in mice have not yet been reported. Therefore, in the present study, following the homeopathic principle of "like cures like", we tested the efficacy of two potencies of a homeopathic drug, Cadmium Sulphoricum (Cad Sulph), in reducing the genotoxic effects of Cadmium chloride in mice.Another objective was to determine the relative efficacy of three administrative modes, i.e. pre-, post- and combined pre and post-feeding of the homeopathic drugs. For this, healthy mice, Mus musculus, were intraperitoneally injected with 0.008% solution of CdCl2 @ 1 ml/100 gm of body wt (i.e. 0.8 mcg/gm of bw), and assessed for the genotoxic effects through such studies as chromosome aberrations (CA), micronucleated erythrocytes (MNE), mitotic index (MI) and sperm head anomaly (SHA), keeping suitable succussed alcohol fed (positive) and CdCl2 untreated normal (negative) controls. The CdCl2 treated mice were divided into 3 subgroups, which were orally administered with the drug prior to, after and both prior to and after injection of CdCl2 at specific fixation intervals and their genotoxic effects were analyzed.ResultsWhile the CA, MNE and SHA were reduced in the drug fed series as compared to their respective controls, the MI showed an apparent increase. The combined pre- and post-feeding of Cad Sulph showed maximum reduction of the genotoxic effects.ConclusionsBoth Cad Sulph-30 and 200 were able to combat cadmium induced genotoxic effects in mice and that combined pre- and post-feeding mode of administration was found to be most effective in reducing the genotoxic effect of CdCl2 followed by the post-feeding mode.
Highlights
Cadmium poisoning in the environment has assumed an alarming problem in recent years
While the chromosome aberrations (CA), micronucleated erythrocytes (MNE) and Sperm headshape abnormality (SHA) were reduced in the drug fed series as compared to their respective controls, the Mitotic Indices (MI) showed an apparent increase
A little deviation has been made in the present experimentation; the homeopathic drug used has been derived from a different salt of cadmium through dilution and succussion as per the homeopathic procedure and it was examined if this drug had any ameliorating effect on the genotoxicity produced by the injection of CdCl2, another cadmium salt
Summary
Cadmium poisoning in the environment has assumed an alarming problem in recent years. In the present study, following the homeopathic principle of "like cures like", we tested the efficacy of two potencies of a homeopathic drug, Cadmium Sulphoricum (Cad Sulph), in reducing the genotoxic effects of Cadmium chloride in mice. Another objective was to determine the relative efficacy of three administrative modes, i.e. pre-, post- and combined pre and post-feeding of the homeopathic drugs. In the present study two microdoses of a potentized homeopathic drug Cadmium Sulphoricum (Cad Sulph) – 30 and 200 were fed to mice either before or after the injection of 0.008% CdCl2 @ 1 ml/100 gm of body weight of mouse, to examine if either or both of these potencies could positively modulate the cytogenetic effects produced by CdCl2. Another objective of the study was to test the comparative efficacies of the three modes of treatment, i.e., pre-, post- and combined pre- and postfeeding of the homeopathic drug in combating sub-acute cadmium poisoning
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
