Comparative efficacy of surface drying and re-drying seed priming in rice: changes in emergence, seedling growth and associated metabolic events

Abstract

In seed priming, seeds are soaked in a solution of low water potential and dried back to their original weight. This study was conducted to evaluate the comparative performance of different seed priming treatments and to investigate whether re-drying is essential or not? Seeds of fine grain aromatic rice (Oryza sativa L.) cultivar Super-Basmati were subjected to hardening, osmohardening with CaCl2 and KCl (ψ s −1.25 MPa) each for 24 h (one cycle), and hydropriming and ascorbate priming (10 mg L−1) for 48 h. Seeds were primed in two sets. In one set after each treatment, seeds were given three surface washings with distilled water and dried back near to original moisture contents with forced air. In the other case, after surface washings with distilled water, seeds were surface dried using blotting paper and sown immediately. All the priming treatments improved the emergence, seedling growth and reserve metabolism. Use of surface drying was more effective for rice seed invigoration, as evident from earlier and synchronized seedling emergence. Moreover, shoot and root length, seedling dry weight, root score, α-amylase activity, soluble sugars and dehydrogenase activity were also improved, although germination percentage and leaf score were similar from both strategies. Results suggested that surface drying, rather than re-drying close to original weight, was more effective, while among the treatments, osmohardening with CaCl2 was the most effective. Increasing rice production with judicious use of water is need of the day, and aerobic rice cultivation is an attractive alternative for this purpose. However, poor and erratic stand establishment is one of the major hindrances in its wide scale adoption. In this regard, seed priming techniques are pragmatic approaches to achieve proper stand establishment in the new rice culture. They help in improving seedling density per unit area under optimal and adverse soil conditions and may be opted to improve the performance of aerobic rice.