Abstract
Motivation/Background:The metastasis of tumor cells consists of steps such as epithelial-mesenchymal transition, transendothelial migration and the formation of metastatic colonization. E-Cadherin and vimentin are main proteins associated with EMT, whereas MMP-9 is associated with migration.
 Method:We aimed to investigate effects boric acid and resveratrol comparatively on metaatatic behaviors on MCF-7. 30μM boric acid and 50μM resveratrol were administered to BA, BA+RES, and RES groups 48hours. Cells stained immunocytochemically by Anti-E-Cadherin, Anti-Vimentin, and Anti-MMP-9 antibodies and H-Score analysis carried out and migration analyzed by woundhealing, morphologically.
 Results andConclusions:It’s been observed that boric acid doesn’t affect the EMT capability of the MCF-7 cells in terms of E-Cadherin and vimentin expression; whereas, it’s affect migration both by decreasing the MMP-9 expression and also by inhibiting migration.
Highlights
Breast cancer is a type of cencer which has the highest mortality rates and is the most common cancer in women Global Cancer Observatory (2021)
We aimed to investigate effects boric acid and resveratrol comparatively on metaatatic behaviors on MCF-7. 30μM boric acid and 50μM resveratrol were administered to Boric acid (BA), boric acid+resveratrol (BA+RES), and RES groups 48hours
Boric acid doses of 10, 30, 50 ve 100 μM to the MCF-7 cells were administered for 48 hours
Summary
Breast cancer is a type of cencer which has the highest mortality rates and is the most common cancer in women Global Cancer Observatory (2021). 10-15% of patients usually have metastasis during the three years following the first treatment Metastasis includes stages such as local invasion (Epithelial-Mesenchymal Transition (EMT)), intravasation, survival in the circulation, extravasation (transendothelial migration (TEM/diapedesis)), the development of micrometastasis and the development of metastatic colonization Reymond et al (2013). Metastasis in cancer cells is a complex process directed by a great number of biochemical and molecular factors. This process and the mechanisms of action are not completely enlightened, the chance to eliminate the disease will arise if the cancer cells can be stopped in the invasion stage
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
