Comparative effect of carotenoid complex from golden neo-life dynamite (GNLD) and carrot ectracted caroteniods on immune parameters in albino wistar rats

Abstract

The immunomodulatory effect of Carotenoid complex from Golden Neo-Life Dynamite (GNLD) and carrot extracted Carotenoid was assessed using 24 albino Wistar rats. The rats were assigned to 4 groups of 6 rats each consisting of group 1(control group treated with distilled water), group 2 (treated with olive oil), group 3 (treated with carrot extract + olive oil) and group 4 (treated with GNLD Carotenoid complex + olive oil). The concentration of carrot extracted Carotenoid and that of the GNLD Carotenoid complex were 559 mg/kg. The animals were sacrificed after 21 days of treatment and whole blood collected for analysis of immune parameters. The parameters included; percentage lymphocytes, percentage eosinophils, percentage monocytes and platelet count. There were significant increases [P < 0.05]; in groups 3 and 4 which received carrot extract and carotenoid complex supplement when compared to group 1 and 2 which received distilled water and olive oil. This supports the claim that carotenoids have immunomodulatory effects. However, there were significant increases [P < 0.05]; in percentage lymphocytes (40.15 +/- 1.33); monocytes (2.50 +/- 1.31);, neutrophils (60.08 +/- 2.06) and platelet count (7.91 +/- 0.53) in group 4 which received carotenoid complex when compared to that of group 3 which received carrot extract; lymphocytes (33.01 +/- 3.20), monocytes (1.52 +/- 0.52), neutrophils (60.12 +/- 1.50) and platelet count (7.81 +/- 0.52). These significant increases[P < 0.05]; show that both carotenoids have immunomodulatory effects; and that the GNLD carotenoid complex which consists of alpha-carotene, beta-carotene, lutein, zeaxanthin, beta-cryptoxanthin, lycopene and alpha-tocopherol is more potent in its immunomodulatory effect compared to the carrot extracted carotenoid which contained mostly alpha-carotene and beta-carotene alone.