Abstract
In the present paper the development and application of a novel somatostatin radioimmunoassay are described. 125I-labeling of Tyr(0)-somatostatin-14 (tyrosin(0)-somatostatin-14) was performed by the iodogen-method and the mono-iodinated peptide was separated by reversed-phase HPLC. As a practical application of the novel RIA, the present work measured the somatostatin concentration parallel with the determination of thrittene content in different rat brain areas. The highest somatostatin concentrations were detected in the cerebellum. The thrittene immunoreactivity in the same brain parts showed a similar tendency. In the present study concluded that thrittene is not created via a specific biosynthetical route, but from the pre-prosomatostatin.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
