Abstract
Abstract In this text, reverse phase HPLC method for separation of bacitracin is described. By selection of optimum chromatographic parameters in isocratic elution, the procedure for quick simple and precise determination of the most important active bacitracin components A, B1 and B2 was obtained. In relation to microbiological determination it was concluded that selective HPLC method gave considerably more useful analytical information on properties and origin of bacitracin samples. Intermethod differences in analysing bacitracin feed grade preparations, can be explained by specific ingredients in this type of samples and less selective microbiological method. Described HPLC method expresses certain advantages over some other published HPLC methods and it is suitable for quantitative and qualitative determinations of bacitracin in pharmaceuticals and feed grade preparations. In stability studies, it is possible to observe, in this way, the appearance of bacitracin F, the main degradation product of bacitracin. It was also found out that component of bacitracin B1 is not homogeneous. Under specific conditions it can be separated in two different submits, and we sign them as B', and B'
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
