Abstract
Equal amounts of Dycal, Cavitec, and N2 were mixed and exposed unset to phosphate-buffer-saline solution for 24 hours at 37 C. To aliquots of the three extracts, 1,4-'4C succinic acid and the pulp tissue of adult male Sprague-Dawley rats were added. Solutions were incubated at 38 C for 15 minutes. The '4CO2 produced by the tissue was measured, and an index of pulpal respiration was determined. Findings were comparable with histologic and cytotoxic cell culture studies that show N2 to be the most toxic. This new method of determining cytotoxicity is relatively simple, rapid, and easy to quantitate.
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