COMPARATIVE CLINICAL, RADIOGRAPHICAL AND HISTOLOGICAL EVALUATION OF CORONAL PULPOTOMY TECHNIQUE WITH PRF AND A NOVEL CROSSLINKED PRF AS AN ALTERNATIVE TO PULPECTOMY FOR PRESERVING THE TOOTH VITALITY IN CONTEXT OF TISSUE ENGINEERING.

Abstract

Background: Removal of infected pulp tissue from the coronal portion leads to preservation of a remaining vital and functioning pulp. Preservation of pulp vitality by pulpotomy is one of the universally accepted treatment procedure with the aid of latest bioactive materials leading to a more conservative approach in the management of teeth involving the pulp based on clinical conditions To comparatively evaluate the . Aim: clinical and radiological and histological outcome of platelet-rich brin (PRF) and the novel crosslinked PRF (C-PRF) developed by us as pulpotomy agent in context of tissue engineering in permanent teeth. 20 permanent mol Materials and Methods: ar teeth from 20 healthy patient aged 18-40 years with permanent molar teeth cariously exposed pulps and diagnosed clinically as irreversible pulpitis were selected for the study. Written consent from the patients was taken. The patients were then randomly divided into 2 groups (n=10) . One group received PRF pulpotomy anther group received our novel crosslinked PRF (C-PRF) pulpotomy. In all patients coronal pulp was removed after preparation of the access cavity with rubber dam under Lignocaine 2 % local anaesthesia. After hemostasis, exposed pulps were covered using PRF membrane with Biodentine or C-PRF with Biodentine, and then all teeth were restored with glass-ionomer cement. Clinical and radiographic evaluation was undertaken at 1 day, 3month and 6 months intervals. The results were analyzed using appropriate statistical tests. After around six months one case of each group third molar teeth was extracted due to positional problem and causation of cheek ulceration. Histological examination of the extracted teeth was done. Statistical Analysis was performed with he Results: lp of Epi Info (TM) 7.2.2.2 EPI INFO is a trademark of the Centers for Disease Control and Prevention (CDC). Test of proportion was used to nd the Standard Normal Deviate (Z) to compare the difference proportions. p<0.05 was taken to be statistically signicant. C-PRF could improve the prognosis Conclusion: of pulpotomy procedures of mature permanent