Comparative characterization of cell products derived from adipose tissue using different systems for the isolation of cellular fractions

Abstract

The use of autologous adipose tissue-derived cell products to restore the morphology and function of organs and tissues affected by the disease have become widespread in regenerative medicine. For the wide application of cell-based products in clinical practice, it is important to develop and implement new devices to automate and standardize the isolation of stromal vascular fraction cells. This work aimed to compare cell products obtained from human adipose tissue using two systems, both registered in the Russian Federation and based on enzymatic and mechanical methods of cell fractions isolation. As clinical material, we used samples of adipose tissue in the form of lipoaspirate obtained from healthy patients. The isolation of cell products was performed according to the instructions for the use of these systems. A comparative analysis of the effectiveness of these systems for the isolation of cell fractions was carried out by evaluating several parameters determined for obtained cell products. The cell product obtained by the enzymatic method is characterized by a high yield of nucleated cells, as well as a high proliferative potential of stem cells isolated from adipose tissue. The cellular product obtained from adipose tissue using the mechanical method is characterized by a low yield of nucleated cells, the presence of a large volume of residual oil, and destroyed connective tissue in the final product. The work shows that the method of adipose tissue processing (enzymatic or mechanical) has a significant effect on the characteristics of the products obtained.