COMPARATIVE CHARACTERISTIC OF EFFECTIVENESS OF SOME DECONTAMINATION FACILITIES USED FOR CARRYING OUT OF FORENSIC MOLECULAR GENETIC EXAMINATION

Abstract

The article is devoted to comparing the performance of disinfectants at different concentrations of a contaminating agent. Contamination of objects can occur at any stage of the study, so effective methods of decontamination should be used to prevent it, which at the same time should be safe for laboratory staff. The purpose of the work is to check the effectiveness of disinfectants while working in biological laboratories and to determine their effects on the genetic information of the cell, the effect on the inhibition of PCR reaction and DNA degradation. The mechanisms of influence on a cell and DNA of chemical solutions are analyzed: Винсепт експресс, Бациллол АФ, Дезактин 0, 2 %, WIP'ANIOS Premium, DNAZap™ PCR DNA Degradation Solutions, 70 % и 96 % ethyl alcohols, and physical methods: ultraviolet radiation, alcohol treatment followed by calcination in the flame of the burner and treatment with bidistilled deionized water. While research the contaminating DNA is represented by the synthetic genomic DNA produced by the Promega company. As a result of the pilot study, DNAZap ™ PCR DNA Degradation Solutions and bidistilled deionized water had the highest decontamination efficiency. Bidistilled deionized water can be used to the fullest in the laboratory if medical waste disposal standards are met. Other methods showed an efficiency of more than 99.98% which also allows them to be used when working in a biological laboratory, following the suggested recommendations. The presented methods of decontamination did not cause inhibition of the PCR reaction. Preserved, after decontamination treatment, DNA for most agents had a low level of degradation. After being treated with ultraviolet radiation, the level of DNA degradation was medium which means that ultraviolet radiation destroys long strands of DNA, leaving strands unbroken up to 200 bp. In addition, increasing the concentration of contaminating DNA did not affect the change in the efficiency of decontamination substances. All the decontaminated substances analyzed can be modified in molecular genetic laboratories.