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Abstract
Anthropogenic activities could expose Fresco lagoon to microbial pollution. The objective of this study was to determine the level of pollution in Fresco lagoon related to fecal contaminations. Two hundred and seventy (270) samples including 216 water and 54 human stools samples from local residents were collected. Escherichia coli was isolated and identified according to classical bacteriology procedure. Strains were characterized by biotyping on API 20E gallery and phylogenetic typing by PCR triplex of Clermont. A set of 392 strains of E. coli was distributed into 18 biotypic profiles. Five biotypes were common to water and human. Classification of all biotypes revealed close relationship between water and human strains because of their repartition in the same groups. Phylogenetic groups A, B1, B2, and D were identified in all strains. Strains belonging to phylogenetic group A were most frequent in water (69.82%) and human stool (44.44%) followed by group B1 in water (24%) and human stool (40.7%). Strains of group B2 were scarce in water (4.4%) and humans (7.41%). The diversity of E. coli biotypes observed in this study revealed animal and human origins of contaminations. A close relationship was found between water and human strains, and the presence of commensal and extraintestinal pathogenic E. coli in all samples could represent a potential reservoir of extraintestinal infections for resident populations.
Highlights
Escherichia coli is a normal inhabitant of lower tract intestinal of warm-blooded animals and humans
E. coli belonging to phylogenetics groups A, biotype 1 (B1), and B2 were isolated from fishes in Fresco lagoon [13]. e presence of E. coli represents a risk of disease if strains are found to be pathogenic. e aim of this present work was to study the relationship between E. coli strains, isolated from water of Fresco lagoon and resident stools, into biotype and phylogenetic groups and assess the likely source of contamination and the presence of potential pathogenic strains in Fresco lagoon
Escherichia coli were detected in all water samples (216), in all sites, and 54 human stool were analyzed. e mean E. coli count varied from 6.29 to 39.62 CFU
Summary
Escherichia coli is a normal inhabitant of lower tract intestinal of warm-blooded animals and humans. Is will no doubt endanger resident aquatic organisms as well as impair the beneficial uses of water. A paucity information exists on extent of biological pollution of Fresco lagoon and its public health implication. Phylogenetic studies showed that there are four main phylogenetic groups of E. coli, designated A, B1, B2, and D [7, 8]. E. coli belonging to phylogenetics groups A, B1, and B2 were isolated from fishes in Fresco lagoon [13]. E presence of E. coli represents a risk of disease if strains are found to be pathogenic. E aim of this present work was to study the relationship between E. coli strains, isolated from water of Fresco lagoon and resident stools, into biotype and phylogenetic groups and assess the likely source of contamination and the presence of potential pathogenic strains in Fresco lagoon E. coli belonging to phylogenetics groups A, B1, and B2 were isolated from fishes in Fresco lagoon [13]. e presence of E. coli represents a risk of disease if strains are found to be pathogenic. e aim of this present work was to study the relationship between E. coli strains, isolated from water of Fresco lagoon and resident stools, into biotype and phylogenetic groups and assess the likely source of contamination and the presence of potential pathogenic strains in Fresco lagoon
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