Comparative Biochemical Studies on the Effect of Nano-Magnetic Particles (Nps) and Graviola Leaves Extract on Adriamycin Induced-Gonadotoxicity in Male Albino Rats

Abd-Elraheim Elshaterˡ,Huda Gedawy,Rana Aliˡ,Mouchira Mohi

doi:10.21608/eajbsc.2018.22663

Abstract

Adriamycin (ADR) is an effective antineoplastic agent commonly used to treat different types of cancer. The use of ADR in clinical chemotherapy is limited due to diverse toxicities, including gonadotoxicity. The present study was designed to evaluate the effect of nano-magnetic particles (NFe3O4) and Graviola leaves extract and (NFe3O4 + Graviola leaves extract) against ADR -induced gonadotoxicity. The rats divided into 5 groups (10 rats per group). The first group (normal g.), the second group received ADR alone (5 mg/kg body weight, i.p.) as (control g.), the third group received ADR followed by NFe3O4 (5 mg/kg body weight, i.p.), the fourth group received ADR followed by Graviola (200mg/kg body weight, orally), and the fifth group received ADR followed by NFe3O4(5 mg/kg body weight, i.p.) and Graviola leaves extract(200mg/kg body weight, orally). Animals were sacrificed 36 days after treatment and sex hormones (Testosterone and F.S.H) in serum. Also, the activities of antioxidants such as (SOD), (CAT), (GSH) and the level of (MDA) as a marker of lipid peroxidation were measured. In the ADR-exposed rats, Testosterone, F.S.H, GSH, SOD and CAT were significantly decreased, while MDA was significantly increased.When compared with normal animals. In the Group NFe3O4, graviola, and (NFe3O4 +graviola), Testosterone, F.S.H, GSH, SOD and CAT enzymes levels were significantly increased, while MDA level was significantly decreased when compared with ADR treated animals. The results showed that there is a possibility that the ethanolic extract of graviola leaves and NFe3O4 ameliorate the gonadotoxicity induced by ADR in rats.

Highlights

The anthracycline antibiotic Adriamycin, is an anticancer drug, it used for the treatment of many types of tumors, and is known to produce reactive oxygen species (ROS) in various tissues
Testosterone and F.S.H concentration in rats treated with NFe3O4, graviola and (NFe3O4 + graviola leaves extract) showed a significant increase as compared to The ADR treated animals, but it was still less than normal animals. (Table1)
While the treatment with NFe3O4,graviola leaves extract or (NFe3O4 + graviola leaves extract) of ADR-exposed rats showed a significant increase in the activities of CAT, SOD and GSH level when compared with control group, but when compared with the normal rats the results indicated that the activities of CAT, SOD and GSH level were improved but not approached to the normal level Table (2).While the ADR treated animals showed a significant increase in the levels of MDA as compared with normal animals

Summary

Introduction

The anthracycline antibiotic Adriamycin, is an anticancer drug, it used for the treatment of many types of tumors, and is known to produce reactive oxygen species (ROS) in various tissues. Though gonadal damage by anticancer drugs like ADR is commonly observed, but it has been relatively less studied compared to the other possible side effects (Sridevi et al, 2012). (Zanetti et al, 2007) Showed that treatments with ADR cause an important decrease in testicular weight resulted in reduced spermatogenic cell number. There is an increased apoptosis in spermatogonia and spermatocytes following ADR treatment (Baumgartner et al, 2004). Acceptance of apoptosis by this medication is one of the soonest indications of genotoxic harm to the grown-up testis. ADR produces persistent damage to the spermatogenic cells as well as the increase in the testicular oxidative stress (Saalu et al, 2010). ADR toxicity can be intervened by collaboration with topoisomerase II, a compound that is plenteous in cells with fast expansion (Brilhante et al, 2012)

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