Abstract
Objective: The aims and objectives of current study was to identify the consequences of over doze Diazepam in biological system. Study Design: It is an experimental Forensic Study in which biological effects of over doze of Diazepam was measured on Albino Rats. Place and Duration of study: Present study was conducted in animal house of Lahore Medical & Dental College. Experimental Albino Rats were purchase from the University of Lahore. Time of duration of this study was 3 month from 20, March 2022 to June 2022. Methodology: Total 20 male albino Rats of approximately 100 gm of weight were selected and divided them into four different groups. In Group A, 5 individuals were control while in group B, group C and group D, there were 5 rats in each group and they were treated orally with 0.2mg/kg, 03mg/kg and 0.4mg/kg Diazepam respectively. Blood pH, serum Creatinine, serum Bicarbonate serum ALT and AST levels were measured after 10, 20 and 30 days of intervals of time. Blood sample were collected from tail in container after centrifugation. Results: Significant (P≤0.05) changes were seen in serum Creatinine, serum Bicarbonate, and serum Alanine transaminase (ALT) and Aspartate aminotransferase (AST) levels of study groups after 30 days than 10 and 20 days. Maximum variations were seen in group-D (1.5±0.01, 42.01±0.02, 47.01±0.04, 49.05±0.01, 7.40±0.02), (1.5±0.01, 47.01±0.02, 101.01±0.04, 139.05±0.01, 7.40±0.02) and (0.6±0.04, 20.10±0.01, 10.02±0.02, 14.01±0.01, 7.35±0.04) as compared with control. Conclusion: In this study it was concluded that over dose of Diazepam caused hepatic toxicity by increasing serum ALT and AST levels and reduces glucose levels. The hepatic toxicity and hypoglycemia may cause death. In study groups the serum Creatinine, serum Bicarbonate levels also showed a significant (P≤0.05) variation in study groups as compared with control. Keywords: Diazepam, Aspartate aminotransferase, Alanine transaminase, Creatinine, Bicarbonate, hypoglycemia
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