Comparative assessment of The Effect of Moringa oleifera Leaf Extract (MLE) and Zeatin on invitro Regeneration Response of Pogostemon cablin Bud Explants

Abstract

Patchouli is one of the essential oil-producing plants which is commonly found in Indonesia, but the productivity tends to decline. Tissue culture technology is one alternative to increase patchouli plant productivity. Tissue culture requires growth regulators in order for the plants to grow optimally. Moringa plants have the potential as a source of growth regulators for tissue culture because Moringa leaves contain the zeatin hormone. Therefore, a comparison test of various concentrations of the zeatin and the moringa leaf extract is needed to determine whether the Moringa leaf extract can be an alternative to the zeatin hormone. This study aimed to investigate the effect of Moringa leaf extract on the growth of patchouli bud explants. The study employed a complete random design (CRD) with the treatment of concentrations of Moringa leaf extract in MS media. Moringa leaf extract was given at 30 grams/L, 40 grams/L, and 50 grams/L, respectively. Meanwhile, the zeatin hormone was given at a concentration of 1.5 ppm, 2 ppm, and 2.5 ppm. Each treatment was repeated four times. Moringa leaves were extracted by maceration method, and patchouli explants were obtained from the 3rd sub-culture of patchouli shoots. From the research results, it can be concluded that (1) the use of Moringa leaf extract can improve the growth of patchouli explants, and (2) the use of 40 grams/L of Moringa leaf extract can be an alternative to substitute the use of 1.5 ppm zeatin hormone.