Comparative Assessment of Hormonal, Echographic and Spectral Parameters in Chronic Endometritis and Chronic Salpingo-Oophoritis

Abstract

The purpose of the study was to assess the effectiveness and clinical significance of the use of spectral analysis of molecular markers for an objective assessment of the clinical course of chronic endometritis and salpingo-oophoritis. Materials and methods. The paper presents survey data of 100 women aged 18-47 years with a diagnosis of chronic endometritis and chronic salpingo-oophoritis. Laboratory methods of blood tests were carried out according to the generally accepted methods when patients were admitted for examination. The assessment of the hormonal status was carried out using standard kits from the company "Immunotec" (Czech Republic) on a radioisotope analyzer "Gamma-800" (Narcotest). All subjects underwent ultrasound of the pelvic organs using the Voluson E8 apparatus, using transabdominal transducers of 11 Hz and 18 Hz and a transvaginal transducer with a frequency of 20 Hz and 31 Hz. To study molecular markers, we used the method of molecular Raman spectroscopy. We used a standard two-beam infrared spectrometer "SPECORD-75 IR" in the frequency range of 4000-400 cm-1. Statistical data processing was carried out using Microsoft Excel 2000 and SPSS 10.0.5 software. Results and discussion. The results of our research showed that the use of hormonal and echographic studies, although they are informative methods in the diagnosis of chronic endometritis and salpingo-oophoritis, are not specific enough. One of the important points of pathogenesis, both for acute and chronic inflammatory diseases of the genitals, is the development of endogenous intoxication. A manifestation of acute inflammation against the background of intoxication is a change in the biosynthesis of "acute phase proteins". Spectral analysis of molecular markers allows detecting chronic endometritis / salpingo-oophoritis with higher accuracy (98%), sensitivity (99%) and specificity (97%). Stretching and bending vibrations of -CO and its derivatives are most intensely manifested. At the same time, depending on the pathological process, a shift in the position of the maxima in the absorption spectra was noted. The magnitude of the shift between the wave numbers obtained in patients with endometritis and salpingo-oophoritis was 1000/1500 cm-1, which is important for the identification and differentiation of components in the mixture. Conclusion. For samples of the spectrum of blood plasma in patients with chronic endometritis, characteristic bands with maxima are located in the region of 1510 cm-1, 1520 cm-1, 1535 cm-1. The absorption spectrum corresponds to 0.13-0.18. In patients with chronic salpingo-oophoritis, 1720cm-1 is characteristic for the 1600/1750cm-1 band, but 3420cm-1 for the 3300/3680cm-1 band. In this case, the absorption of waves during this process has significant differences – 0.16-0.25 for the first band and 0.06-0.20 for the second, on average 0.11