Abstract
Crinum asiaticum L. (family: Amaryllidaceae) is a widely used medicinal plant species having great ethnomedicinal values. The present study aimed to evaluate in vitro antioxidant and antimicrobial potentials of the leaf extracts of C. asiaticum with their phenolics content. The total antioxidant assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, and ferric ion reducing antioxidant power (FRAP) assays of the leaf crude aqueous extract and the five successive extract fractions were analyzed to evaluate their antioxidant potential. The antimicrobial potential was investigated against two gram-negative and one gram-positive bacteria strain by the disc diffusion method. The aqueous fraction of C. asiaticum (CaAq) showed relatively higher antimicrobial potentials than the crude aqueous extract (CaLAE), in terms of zone of inhibition, indicating the fact that serial extraction procedure can more successfully concentrate the specific bioactive components. Along with CaAq and CaLAE, CaEA fraction (ethyl acetate extract fraction) also exhibits antimicrobial activities probably due to the presence of terpenoids, phenolics, and flavonoids. It might be concluded that antioxidants and antimicrobial agents are available in the leaves of C. asiaticum where water and ethyl acetate might be the effective solvent systems to extract antimicrobial and antioxidant agents respectively.
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