Abstract
The antigencity of different cholera vaccines was compared by active mouse protection tests, using hog gastric mucin and ferric ammonium citrate as virulence-enhancing agents. The results were evaluated by analysis of variance (parallel line assay). No significant difference was found between the vaccines with either mucin or ferric ammonium citrate used as virulence-enhancing agents. However, the marked difference found in the relative potencies and, especially in the 95% fiducial limits, served as a reminder of the importance of careful checking and mathematical-statistical evaluation of all parameters in each case of vaccine standardization.
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