Abstract
The rnfABCDE genes in Rhodobacter capsulatus are essential for diazotrophic growth under illuminated conditions. They locate near fdxN gene whose product is a major electron donor to nitrogenase. Since disruption of rnf genes conferred metronidazole resistance under pseudo-diazotrophic conditions and two of the rnf products, RnfB and RnfC, are supposed to hold iron-sulfur clusters, the genes are expected to encode some ferredoxin reductase. Because most of rnf products are highly hydrophobic it is hypothesized that rnf products constitute a membrane protein complex. In this study, we have 1) experimentally investigated their presence at chromatophore membranes, 2) made a prediction of enzymatic function and 3) collected data that the rnf products also serve for nitrogen fixation in darkness and that their homologs present in non-diazotrophs.
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