Abstract
Many species of the genus Kalanchoe are important horticultural plants. They have evolved the Crassulacean acid metabolism (CAM) photosynthetic pathway to allow them to be better adapted to dry environments. Despite their importance, it is still debating whether Kalanchoe is monophyletic, and understanding the past diversification of this genus requires a tremendous amount of effort and work being devoted to the studies of morphological and molecular characters of this genus. However, molecular information, plastic sequence data, in particular, reported on Kalanchoe species is scarce, and this has posed a great challenge in trying to interpret the evolutionary history of this genus. In this study, plastomes of the five Kalanchoe species, including Kalanchoe daigremontiana, Kalanchoe delagoensis, Kalanchoe fedtschenkoi, Kalanchoe longiflora, and Kalanchoe pinnata, were sequenced and analyzed. The results indicate that the five plastomes are comparable in size, guanine-cytosine (GC) contents and the number of genes, which also demonstrate an insignificant difference in comparison with other species from the family Crassulaceae. About 224 simple sequence repeats (SSRs) and 144 long repeats were identified in the five plastomes, and most of these are distributed in the inverted repeat regions. In addition, highly divergent regions containing either single nucleotide polymorphism (SNP) or insertion or deletion (InDel) mutations are discovered, which could be potentially used for establishing phylogenetic relationships among members of the Kalanchoe genus in future studies. Furthermore, phylogenetic analyses suggest that Bryophyllum should be placed into one single genus as Kalanchoe. Further genomic analyses also reveal that several genes are undergone positive selection. Among them, 11 genes are involved in important cellular processes, such as cell survival, electron transfer, and may have played indispensable roles in the adaptive evolution of Kalanchoe to dry environments.
Highlights
The genus Kalanchoe Adans. in the family Crassulaceae comprises 144 species (Kubitzki, 2007) and are mainly distributed in the tropical regions of Africa and Asia
The size, structure, GC contents, and the number of genes are similar to other Crassulaceaen plastid genomes
Several simple sequence repeats (SSRs) and divergent regions were detected, and they were used in interpreting the evolutionary relationship of the five species
Summary
The genus Kalanchoe Adans. in the family Crassulaceae comprises 144 species (Kubitzki, 2007) and are mainly distributed in the tropical regions of Africa and Asia. In the family Crassulaceae comprises 144 species (Kubitzki, 2007) and are mainly distributed in the tropical regions of Africa and Asia. Members of this genus typically have succulent leaves, pendulous or erect flowers, and eight stamens inserted in the middle or at the base of the tubular corolla (Baldwin, 1938). When the genus Kalanchoe was first published by Michel Adanson (1727–1806), its taxonomical status with Bryophyllum Salisb. With advances in molecular technology, the taxonomical status of Kalanchoe has been revisited, and progress has been made in this area in recent years (Van Ham and Hart, 1998; Gehrig et al, 2001; Gontcharova and Gontcharov, 2009; Chernetskyy, 2013; Smith and Figueiredo, 2018). Uncertainty remains, and more work needs to be done to fully elucidate the taxonomical properties and the phylogenetic history of Kalanchoe
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