Comparative analysis on the genetic diversity of cultured and wild silver pomfret populations based on mtD-loop and <I>CO</I>I gene

Abstract

The genetic diversity of cultured and wild silver pomfret (Pampus argenteus) populations was analyzed based on mtD-loop and COI gene. The results showed that the average A,T,C and G contents in D-loop were 40.00%,30.55%,16.75% and 12.70%,respectively,and the contents of A + T were 70.55%,higher than those of G + C. In the COI gene,the average A,T,C and G contents in D-loop gene were 25.85%,33.90%,21.30% and 18.85%,respectively,and the contents of A + T were 59.75%,also higher than those of G + C. The total variable sites,number of haplotypes (h),haplotype diversity (Hd),nucleotide diversity (π) and mean pairwise nucleotide differences (k) of two populations based on D-loop were 19,15,0.895,0.007 and 2.505,respectively. The same parameters based on COI gene were 33,17,0.713,0.004 and 2.239,respectively. Based on mtD-loop and COI gene,the genetic diversity of cultured population was lower than that of wild population. The Hd of cultured population based on mtD-loop and COI gene were 0.562 and 0.571,respectively. And the Hd of wild population were 0.891 and 0.801,respectively. The AMOVA analysis based on D-loop showed that the significant genetic divergence existed in cultured and wild populations,while there was no significant genetic divergence when it was analyzed based on COI gene. In conclusion,both D-loop and COI gene were effective molecular markers for analyzing the genetic diversity of silver pomfret population,while the sensitivity of D-loop in detecting the genetic diversity among populations was higher than that of COI gene.